Boltzmann Populations in a Two-State Landscape
Change the relative well depths, the barrier height, and the thermal energy. Watch molecules hop between two conformational states and compare the measured population with the Boltzmann prediction.
Measured PB
0.50
Boltzmann PB
0.50
kA→B
0.27
kB→A
0.27
Energy Landscape and Hopping
Population and Boltzmann Prediction
Population Relaxation in Time
Main idea
Well depths determine equilibrium populations.
Barrier height determines how fast the system approaches equilibrium.